Genome-Wide Characterization of Shi-Related Sequence Gene Family and Its Roles in Response to Zn<sup>2+</sup> Stress in Cucumber
Xinhui Zhang,
Bilal Ahmad,
Shuang Zeng,
Yuhan Lan,
Xin Hu,
Lingling Fu,
Tian Hu,
Jinhua Li,
Xingguo Zhang,
Yu Pan,
Dan Du
Affiliations
Xinhui Zhang
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Bilal Ahmad
National Key Laboratory of Tropical Crop Breeding, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
Shuang Zeng
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Yuhan Lan
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Xin Hu
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Lingling Fu
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Tian Hu
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Jinhua Li
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Xingguo Zhang
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Yu Pan
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Dan Du
Key Laboratory of Agricultural Biosecurity and Green Production in the Upper Reaches of the Yangtze River, Ministry of Education/College of Horticulture and Landscape Architecture, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
Shi-related sequence (SRS) proteins, which consist of the RING-like zinc finger domain and IGGH domain, are plant-specific transcription factors that have been well-studied in several plant species. However, information about SRS genes and their roles in cucumber (Cucumis sativus L.) is limited. Therefore, we performed detailed bioinformatic analysis of the SRS gene family, including gene numbers and positions, genes structures, conserved motif distribution patterns, phylogenetic analysis, and promoter cis-element analysis. Eight SRS genes were identified in cucumber and distributed on all seven cucumber chromosomes. SRS genes are conserved in plants and divided into two groups in cucumber based on their protein sequence. In silico analysis predicted that most genes may function in response to abiotic stresses and phytohormones. Gene ontology analysis predicted the possible involvement of genes in development and reproduction, and DNA and protein binding on a molecular level. Furthermore, the differential expression pattern of SRS genes in leaf, stem and root under the condition of Zn2+ stress suggested their roles in response to Zn2+ stress. Furthermore, our metal tolerance assay suggested that CsSRS2 and CsSRS5 mediated enhanced tolerance to Zn2+ stress in Escherichia coli cells. Our study provides a foundation for the functional study of SRS genes in cucumber.
