Comparative Assessment of <i>Yersinia pestis</i> Strains on the Level of Plague Microbe Capsular Antigen Production

Abstract

Yersinia pestis recombinant strain KM277 (EV11MpFSK-3) Kmr is a potential producer-strain of capsular antigen (F1), since its productive properties and immunochemical activity of F1-antigen, synthesized by it, are higher than in Y. pestis EV and its derivatives. The apparent advantage of the Y. pestis KM277 strain is the ability to synthesize F1 antigen at 28 °C, and the absence of its own plague-microbe plasmids in cells. Worked out is the scheme of scaled cultivation of the recombinant strain and obtainment of capsular antigen preparation.

Keywords

• <i>yersinia pestis</i>
• capsular antigen (f1)
• cultivation