Frontiers in Cell and Developmental Biology (Jan 2022)
Down-Regulating HAUS6 Suppresses Cell Proliferation by Activating the p53/p21 Pathway in Colorectal Cancer
- Aling Shen,
- Aling Shen,
- Liya Liu,
- Liya Liu,
- Yue Huang,
- Yue Huang,
- Zhiqing Shen,
- Zhiqing Shen,
- Meizhu Wu,
- Meizhu Wu,
- Xiaoping Chen,
- Xiaoping Chen,
- Xiangyan Wu,
- Xiangyan Wu,
- Xiaoying Lin,
- Xiaoying Lin,
- Youqin Chen,
- Youqin Chen,
- Youqin Chen,
- Li Li,
- Ying Cheng,
- Ying Cheng,
- Jianfeng Chu,
- Jianfeng Chu,
- Thomas J. Sferra,
- Lihui Wei,
- Lihui Wei,
- Qunchuan Zhuang,
- Qunchuan Zhuang,
- Jun Peng,
- Jun Peng
Affiliations
- Aling Shen
- Academy of Integrative Medicine, Fuzhou, China
- Aling Shen
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Liya Liu
- Academy of Integrative Medicine, Fuzhou, China
- Liya Liu
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Yue Huang
- Academy of Integrative Medicine, Fuzhou, China
- Yue Huang
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Zhiqing Shen
- Academy of Integrative Medicine, Fuzhou, China
- Zhiqing Shen
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Meizhu Wu
- Academy of Integrative Medicine, Fuzhou, China
- Meizhu Wu
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Xiaoping Chen
- Academy of Integrative Medicine, Fuzhou, China
- Xiaoping Chen
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Xiangyan Wu
- Academy of Integrative Medicine, Fuzhou, China
- Xiangyan Wu
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Xiaoying Lin
- Academy of Integrative Medicine, Fuzhou, China
- Xiaoying Lin
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Youqin Chen
- Academy of Integrative Medicine, Fuzhou, China
- Youqin Chen
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Youqin Chen
- Department of Pediatrics, Case Western Reserve University School of Medicine, Rainbow Babies and Children’s Hospital, Cleveland, OH, United States
- Li Li
- Department of Health Management, Fujian Provincial Hospital, Fuzhou, China
- Ying Cheng
- Academy of Integrative Medicine, Fuzhou, China
- Ying Cheng
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Jianfeng Chu
- Academy of Integrative Medicine, Fuzhou, China
- Jianfeng Chu
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Thomas J. Sferra
- Department of Health Management, Fujian Provincial Hospital, Fuzhou, China
- Lihui Wei
- Academy of Integrative Medicine, Fuzhou, China
- Lihui Wei
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Qunchuan Zhuang
- Academy of Integrative Medicine, Fuzhou, China
- Qunchuan Zhuang
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- Jun Peng
- Academy of Integrative Medicine, Fuzhou, China
- Jun Peng
- Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, China
- DOI
- https://doi.org/10.3389/fcell.2021.772077
- Journal volume & issue
-
Vol. 9
Abstract
Background: HAUS6 participates in microtubule-dependent microtubule amplification, but its role in malignancies including colorectal cancer (CRC) has not been explored. We therefore assessed the potential oncogenic activities of HAUS6 in CRC.Results: HAUS6 mRNA and protein expression is higher in CRC tissues, and high HAUS6 expression is correlated with shorter overall survival in CRC patients. HAUS6 knockdown in CRC cell lines suppressed cell growth in vitro and in vivo by inhibiting cell viability, survival and arresting cell cycle progression at G0/G1, while HAUS6 over-expression increased cell viability. We showed that these effects are dependent on activation of the p53/p21 signalling pathway by reducing p53 and p21 degradation. Moreover, combination of HAUS6 knockdown and 5-FU treatment further enhanced the suppression of cell proliferation of CRC cells by increasing activation of the p53/p21 pathway.Conclusion: Our study highlights a potential oncogenic role for HAUS6 in CRC. Targeting HAUS6 may be a promising novel prognostic marker and chemotherapeutic target for treating CRC patients.
Keywords
