Untargeted Metabolomics Approach Correlated Enniatin B Mycotoxin Presence in Cereals with Kashin–Beck Disease Endemic Regions of China
Danlei Sun,
Camille Chasseur,
Françoise Mathieu,
Jessica Lechanteur,
Pierre Van Antwerpen,
Joanne Rasschaert,
Véronique Fontaine,
Cédric Delporte
Affiliations
Danlei Sun
Unit of Microbiology, Bioorganic and Macromolecular Chemistry, Faculty of Pharmacy, Université libre de Bruxelles (ULB), 1050 Brussels, Belgium
Camille Chasseur
Unit of Microbiology, Bioorganic and Macromolecular Chemistry, Faculty of Pharmacy, Université libre de Bruxelles (ULB), 1050 Brussels, Belgium
Françoise Mathieu
Kashin–Beck Disease Fund, 6953 Forrieres, Belgium
Jessica Lechanteur
Laboratory of Bone and Metabolic Biochemistry, Faculty of Medicine, Université libre de Bruxelles (ULB), 1070 Brussels, Belgium
Pierre Van Antwerpen
Unit of Pharmacognosy, Bioanalysis and Drug Discovery Unit & Analytical Platform of the Faculty of Pharmacy (APFP), Faculty of Pharmacy, Université libre de Bruxelles (ULB), 1050 Brussels, Belgium
Joanne Rasschaert
Laboratory of Bone and Metabolic Biochemistry, Faculty of Medicine, Université libre de Bruxelles (ULB), 1070 Brussels, Belgium
Véronique Fontaine
Unit of Microbiology, Bioorganic and Macromolecular Chemistry, Faculty of Pharmacy, Université libre de Bruxelles (ULB), 1050 Brussels, Belgium
Cédric Delporte
Unit of Pharmacognosy, Bioanalysis and Drug Discovery Unit & Analytical Platform of the Faculty of Pharmacy (APFP), Faculty of Pharmacy, Université libre de Bruxelles (ULB), 1050 Brussels, Belgium
Kashin–Beck disease (KBD) is a multifactorial endemic disease that only occurs in specific Asian areas. Mycotoxin contamination, especially from the Fusarium spp., has been considered as one of the environmental risk factors that could provoke chondrocyte and cartilage damage. This study aimed to investigate whether new mycotoxins could be identified in KBD-endemic regions as a potential KBD risk factor. This was investigated on 292 barley samples collected in Tibet during 2009–2016 and 19 wheat samples collected in Inner Mongolia in 2006, as control, from KBD-endemic and non-endemic areas. The LC-HRMS(/MS) data, obtained by a general mycotoxin extraction technic, were interpreted by both untargeted metabolomics and molecular networks, allowing us to identify a discriminating compound, enniatin B, a mycotoxin produced by some Fusarium spp. The presence of Fusarium spp. DNA was detected in KBD-endemic area barley samples. Further studies are required to investigate the role of this mycotoxin in KBD development in vivo.
