Assessing the ability of polysaccharides extracted from date palm fruit to salvage Wistar rats from cisplatin-linked hepatic damage

Abstract

Background: Cisplatin is a platinum-based chemotherapeutic drug utilized in the treatment of many solid-tissue cancers; it is associated with several organ toxicities. For ages, Phoenix Dactylifera, known as the '' large jujube '' or '' dà zǎo,'' in Chinese traditional medicine, has been employed for several medicinal applications. The present study assesses the role of Date Fruit Polysaccharides (DFP) in cisplatin-induced liver injury Method: Rats were intraperitoneally treated with a single therapeutic dose of cisplatin (5 mg/kg body weight) and then orally treated daily with or without 50/100 mg/kg body weight of DFP for 7 successive days.The salvaging effects of DFP were assessed on Cisplatin-induced hepatic damage, by investigating the hepatic function markers, oxidative stress, and pro-inflammatory biomarkers, with histopathological assessment of the liver morphology by hematoxylin/eosin stain. To elucidate the contents, functional groups, and antioxidative potentials of DFP, chromatographic, spectroscopic, and in vitro antioxidative assays were analysed. Results: Exposure to a single dose of cisplatin led to a considerable escalation in the tested hepatic function biomarkers (ALP and ALT), with an associated upsurge in levels/activities of malondialdehyde, cytokines, myeloperoxidase and a significant drop in the level of GSH (P < 0.05) in the liver as compared to the control. Moreover, there is also an obvious decline in antioxidant enzymes (catalase, SOD and GPx) activities. Contrarily, post-treatment with DFP significantly (P < 0.05) inhibited the heightened hepatic function markers, lipid peroxidation, inflammation, and oxidative stress dose-dependently. Analysis of chemical constituents, functional groups, and in vitro activities demonstrated important monosaccharides and antioxidative properties of DFP. Conclusion: This study shows the ability of DFP to serve as a probable salvaging agent in hepatic damage associated with cisplatin treatment.

Keywords

• Cisplatin
• Hepatic damage
• Oxidative stress, Phoenix dactylifera
• Polysaccharides
• Hepatoprotective