International Journal of Nanomedicine (2020-01-01)

Incorporation of ROS-Responsive Substance P-Loaded Zeolite Imidazolate Framework-8 Nanoparticles into a Ca2+-Cross-Linked Alginate/Pectin Hydrogel for Wound Dressing Applications

  • Zhu Y,
  • Yao Z,
  • Liu Y,
  • Zhang W,
  • Geng L,
  • Ni T

Journal volume & issue
Vol. Volume 15
pp. 333 – 346

Abstract

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Yiming Zhu, 1 Zuochao Yao, 2 Yushu Liu, 3 Wen Zhang, 2 Lele Geng, 2 Tao Ni 2 1Department of General Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 2Department of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Huangpu, Shanghai, China; 3Department of Burns and Plastic Surgery, Binzhou Medical University Hospital, Binzhou City, Shandong Province, ChinaCorrespondence: Tao NiDepartment of Plastic and Reconstructive Surgery, Shanghai Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, No. 639 Zhizaoju Road, Huangpu District, Shanghai 200011, People’s Republic of ChinaEmail [email protected]: Wound healing, especially of extensive full-thickness wounds, is one of the most difficult problems in clinical studies. In this study, we prepared a novel substance P (SP)-delivery system using zeolite imidazolate framework-8 (ZIF-8) nanoparticles.Methods: We synthesized ZIF-8 nanoparticles using a modified biomimetic mineralization method. We then coated SP-loaded ZIF-8 nanoparticles ([email protected]) with polyethylene glycol-thioketal (PEG-TK) to fabricate [email protected] nanoparticles, and encapsulated them in injectable hydrogel composed of sodium alginate and pectin and cross-linked using calcium chloride. The final hydrogel wound dressing containing [email protected] nanoparticles was called [email protected]@CA.Results: The fabricated ZIF-8 nanoparticles had high SP-loading efficiency. SP-release assay showed that the [email protected] nanoparticles maintained drug activity and showed responsive release under stimulation by reactive oxygen species. The [email protected] nanoparticles promoted proliferation of human dermal fibroblasts, up-regulated expression levels of inflammation-related genes in macrophages, and exhibited favorable cytocompatibility in vitro. Full-thickness excision wound models in vivo confirmed that [email protected]@CA dressings had excellent wound-healing efficacy by promoting an early inflammatory response and subsequent M2 macrophage polarization in the wound-healing process.Conclusion: In conclusion, these findings indicated that [email protected]@CA dressings might be useful for wound dressing applications in the clinic.Keywords: substance P, metal-organic framework, nanoparticles, hydrogel, wound healing

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