Generation of Gαi knock-out HEK293 cells illuminates Gαi-coupling diversity of GPCRs

Yuki Ono; Kouki Kawakami; Gaku Nakamura; Satoru Ishida; Junken Aoki; Asuka Inoue

doi:10.1038/s42003-023-04465-2

Communications Biology (Jan 2023)

Generation of Gαi knock-out HEK293 cells illuminates Gαi-coupling diversity of GPCRs

Yuki Ono,
Kouki Kawakami,
Gaku Nakamura,
Satoru Ishida,
Junken Aoki,
Asuka Inoue

Affiliations

Yuki Ono: Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University
Kouki Kawakami: Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University
Gaku Nakamura: Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University
Satoru Ishida: Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University
Junken Aoki: Department of Health Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo
Asuka Inoue: Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University

DOI: https://doi.org/10.1038/s42003-023-04465-2
Journal volume & issue: Vol. 6, no. 1
pp. 1 – 9

Abstract

Read online

A HEK293 cell line lacking all Gαi subunits is generated which enables the measurement of coupling between a GPCR and individual Gαi subunits upon transient re-expression of a specific Gαi subunit.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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