Emerging Infectious Diseases (Feb 2004)

Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

  • Junhui Zhai,
  • Thomas Briese,
  • Erhei Dai,
  • Xiaoyi Wang,
  • Xin Pang,
  • Zongmin Du,
  • Haihong Liu,
  • Jin Wang,
  • Hongxia Wang,
  • Zhaobiao Guo,
  • Zeliang Chen,
  • Lingxiao Jiang,
  • Dongsheng Zhou,
  • Yanping Han,
  • Omar Jabado,
  • Gustavo Palacios,
  • W. Ian Lipkin,
  • Ruifu Yang

DOI
https://doi.org/10.3201/eid1002.030799
Journal volume & issue
Vol. 10, no. 2
pp. 300 – 303

Abstract

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During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.

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