Differences in Extracellular NAD<sup>+</sup> and NMN Metabolism on the Surface of Vascular Endothelial Cells
Patrycja Jablonska,
Paulina Mierzejewska,
Marta Tomczyk,
Patrycja Koszalka,
Marika Franczak,
Ada Kawecka,
Barbara Kutryb-Zajac,
Alicja Braczko,
Ryszard T. Smolenski,
Ewa M. Slominska
Affiliations
Patrycja Jablonska
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Paulina Mierzejewska
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Marta Tomczyk
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Patrycja Koszalka
Institute of Medical Biotechnology and Experimental Oncology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Medical University of Gdansk, 80-211 Gdansk, Poland
Marika Franczak
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Ada Kawecka
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Barbara Kutryb-Zajac
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Alicja Braczko
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Ryszard T. Smolenski
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
Ewa M. Slominska
Department of Biochemistry, Medical University of Gdansk, 80-211 Gdansk, Poland
The disruption of the metabolism of extracellular NAD+ and NMN may affect related signaling cascades and pathologies, such as cardiovascular or respiratory system diseases. We aimed to study NAD+ and NMN hydrolysis on surface endothelial cells of diverse origins and with genetically modified nucleotide catabolism pathways. We tested lung endothelial cells isolated from C57BL/6 J wild-type (WT) and C57BL/6 J CD73 knockout (CD73 KO) mice, the transfected porcine iliac artery endothelial cell line (PIEC) with the human E5NT gene for CD73 (PIEC CD73), and a mock-transfected control (PIEC MOCK), as well as HMEC-1 and H5V cells. Substrate conversion into the product was followed by high-performance liquid chromatography (HPLC). We showed profound differences in extracellular NAD+ and NMN metabolism related to the vessel origin, species diversity, and type of culture. We also confirmed the involvement of CD38 and CD73 in NAD+ and NMN cleavage.
