Raman microspectroscopy and Raman imaging reveal biomarkers specific for thoracic aortic aneurysms

Kaori Sugiyama; Julia Marzi; Julia Alber; Eva M. Brauchle; Masahiro Ando; Yoshito Yamashiro; Bhama Ramkhelawon; Katja Schenke-Layland; Hiromi Yanagisawa

Cell Reports Medicine (May 2021)

Raman microspectroscopy and Raman imaging reveal biomarkers specific for thoracic aortic aneurysms

Kaori Sugiyama,
Julia Marzi,
Julia Alber,
Eva M. Brauchle,
Masahiro Ando,
Yoshito Yamashiro,
Bhama Ramkhelawon,
Katja Schenke-Layland,
Hiromi Yanagisawa

Affiliations

Kaori Sugiyama: Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan; Institute for Advanced Research of Biosystem Dynamics, Research Institute for Science and Engineering, Waseda University, Tokyo, Japan
Julia Marzi: Department of Women’s Health, Research Institute of Women’s Health, Eberhard Karls University, Tübingen, Germany; NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany; Cluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies,” University of Tübingen, Tübingen, Germany
Julia Alber: Department of Women’s Health, Research Institute of Women’s Health, Eberhard Karls University, Tübingen, Germany
Eva M. Brauchle: Department of Women’s Health, Research Institute of Women’s Health, Eberhard Karls University, Tübingen, Germany; NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany; Cluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies,” University of Tübingen, Tübingen, Germany
Masahiro Ando: Research Organization for Nano & Life Innovation, Waseda University, Tokyo, Japan; Japan Science and Technology Agency, PRESTO, Saitama, Japan
Yoshito Yamashiro: Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan
Bhama Ramkhelawon: Division of Vascular Surgery, Department of Surgery and Department of Cell Biology, New York University Langone Health, New York, NY, USA
Katja Schenke-Layland: Department of Women’s Health, Research Institute of Women’s Health, Eberhard Karls University, Tübingen, Germany; NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany; Cluster of Excellence iFIT (EXC 2180) “Image-Guided and Functionally Instructed Tumor Therapies,” University of Tübingen, Tübingen, Germany; Department of Medicine/Cardiology, Cardiovascular Research Laboratories, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA; Corresponding author
Hiromi Yanagisawa: Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, Tsukuba, Japan; Department of Basic Medical Science, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan; Corresponding author

Journal volume & issue: Vol. 2, no. 5
p. 100261

Abstract

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Summary: Aortic rupture and dissection are life-threatening complications of ascending thoracic aortic aneurysms (aTAAs), and risk assessment has been largely based on the monitoring of lumen size enlargement. Temporal changes in the extracellular matrix (ECM), which has a critical impact on aortic remodeling, are not routinely evaluated, and cardiovascular biomarkers do not exist to predict aTAA formation. Here, Raman microspectroscopy and Raman imaging are used to identify spectral biomarkers specific for aTAAs in mice and humans by multivariate data analysis (MVA). Multivariate curve resolution-alternating least-squares (MCR-ALS) combined with Lasso regression reveals elastic fiber-derived (Ce1) and collagen fiber-derived (Cc6) components that are significantly increased in aTAA lesions of murine and human aortic tissues. In particular, Cc6 detects changes in amino acid residues, including phenylalanine, tyrosine, tryptophan, cysteine, aspartate, and glutamate. Ce1 and Cc6 may serve as diagnostic Raman biomarkers that detect alterations of amino acids derived from aneurysm lesions.

Published in Cell Reports Medicine

ISSN: 2666-3791 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General)
Website: https://www.cell.com/cell-reports-medicine

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