Sensor Based on PZT Ceramic Resonator with Lateral Electric Field for Immunodetectionof Bacteria in the Conducting Aquatic Environment †

Abstract

A biological sensor for detection and identification of bacterial cells, including a resonator with a lateral electric field based on PZT ceramics was experimentally investigated. For bacterial immunodetection the frequency dependencies of the electric impedance of the sensor with a suspension of microbial cells were measured before and after adding the specific antibodies. It was found that the addition of specific antibodies to a suspension of microbial cells led to a significant change in these frequency dependencies due to the increase in the conductivity of suspension. The analysis of microbial cells was carried out in aqueous solutions with a conductivity of 4.5–1000 μS/cm, as well as in the tap and drinking water. The detection limit of microbial cells was found to be 103 cells/mL and the analysis time did not exceed 4 min. Experiments with non-specific antibodies were also carried out and it was shown that their addition to the cell suspension did not lead to a change in the analytical signal of the sensor. This confirms the ability to not only detect, but also identify bacterial cells in suspensions.

Keywords

• immunodetection of microbial cells in aqueous medium
• PZT resonator with lateral electric field
• electrical impedance
• antibodies (Abs)