Ultra-fast force-clamp spectroscopy data on the interaction between skeletal muscle myosin and actin
Manuela Maffei,
Diego Beneventi,
Monica Canepari,
Roberto Bottinelli,
Francesco Saverio Pavone,
Marco Capitanio
Affiliations
Manuela Maffei
Department of Molecular Medicine, University of Pavia, Pavia, Italy
Diego Beneventi
LENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy
Monica Canepari
Department of Molecular Medicine, University of Pavia, Pavia, Italy
Roberto Bottinelli
Department of Molecular Medicine, University of Pavia, Pavia, Italy
Francesco Saverio Pavone
LENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy; Department of Physics and Astronomy, University of Florence, Via Sansone 1, 50019 Sesto Fiorentino, Italy; National Institute of Optics–National Research Council, Largo Fermi 6, 50125 Florence, Italy
Marco Capitanio
LENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy; Department of Physics and Astronomy, University of Florence, Via Sansone 1, 50019 Sesto Fiorentino, Italy; Corresponding author. LENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019 Sesto Fiorentino, Italy.
Ultrafast force-clamp spectroscopy is a single molecule technique based on laser tweezers with sub-millisecond and sub-nanometer resolution. The technique has been successfully applied to investigate the rapid conformational changes that occur when a myosin II motor from skeletal muscle interacts with an actin filament. Here, we share data on the kinetics of such interaction and experimental records collected under different forces [1]. The data can be valuable for researchers interested in the mechanosensitive properties of myosin II, both from an experimental and modeling point of view. The data is related to the research article “ultrafast force-clamp spectroscopy of single molecules reveals load dependence of myosin working stroke” [2]. Keywords: Optical tweezers, Force-clamp spectroscopy, Myosin, Single molecule biophysics
