OncoTargets and Therapy (Mar 2020)

Adequate Lymph Node Assessments and Investigation of Gut Microorganisms and Microbial Metabolites in Colorectal Cancer

  • Han S,
  • Wu W,
  • Da M,
  • Xu J,
  • Zhuang J,
  • Zhang L,
  • Zhang X,
  • Yang X

Journal volume & issue
Vol. Volume 13
pp. 1893 – 1906

Abstract

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Shuwen Han,1 Wei Wu,2 Miao Da,3 Jiamin Xu,4 Jing Zhuang,4 Longqi Zhang,5 Xiaoxiang Zhang,6 Xi Yang7 1Department of Oncology, Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Huzhou, Zhejiang, People’s Republic of China; 2Department of Gastroenterology, Huzhou Central Hospital, Huzhou, Zhejiang, People’s Republic of China; 3Department of Nursing, Huzhou Third Municipal Hospital, Huzhou, Zhejiang, People’s Republic of China; 4Graduate School of Nursing, Huzhou University, Huzhou, Zhejiang, People’s Republic of China; 5Department of Infectious Disease, Huzhou Central Hospital, Huzhou, Zhejiang, People’s Republic of China; 6Department of Clinical Examination, Huzhou Central Hospital, Huzhou, Zhejiang, People’s Republic of China; 7Department of Oncology, Huzhou Central Hospital, Huzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Xi YangDepartment of Oncology, Huzhou Central Hospital, Huzhou, Zhejiang, People’s Republic of ChinaTel +86 57 2202 3301Email [email protected]: To analyze the lymph node metastasis status and prognosis in CRCs and to investigate the gut microorganisms and microbial metabolites at different lymph node stages.Methods: The Surveillance, Epidemiology, and End Results (SEER) database and STAT software were used to analyze the clinical features and lymph node metastasis. Bacterial 16S V3-V4 and fungal ITS V3-V4 ribosomal RNA genes were sequenced in 53 stool samples and gas chromatography/mass spectrometry (GS/MS) was performed to detect the microbial metabolites in 48 stool samples from CRC patients.Results: A higher number of lymph node metastases predicted a poor prognosis. Inadequate evaluation of lymph nodes affects the accuracy of prognostic assessments. We constructed a nomogram model for the assessment of prognostic factors. There were multiple characteristic bacteria identified, including Akkermansia, Megamonas, Dialister, etc., and fungi, including Penicillium, Filobasidium, Debaryomyces, etc. A total of 27 characteristic microbial metabolites in different lymph node metastasis status were also identified.Conclusion: Gut microorganisms and microbial metabolites may provide reference and guidance for the adequate lymph node assessments (ALNA) in CRC.Keywords: microbiome, metabolites, lymph node, metastasis, colorectal cancer

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