Making Bunyaviruses Talk: Interrogation Tactics to Identify Host Factors Required for Infection

Abstract

The identification of host cellular genes that act as either proviral or antiviral factors has been aided by the development of an increasingly large number of high-throughput screening approaches. Here, we review recent advances in which these new technologies have been used to interrogate host genes for the ability to impact bunyavirus infection, both in terms of technical advances as well as a summary of biological insights gained from these studies.

Keywords

• bunyavirus
• high-throughput screening
• host-pathogen interaction
• haploid genetic screening
• RNAi screening
• yeast two-hybrid
• affinity purification mass spectrometry