Anticandidal and antibiofilm activity of Artocarpus lakoocha extract

Abstract

This study aimed to investigate the anticandidal and antibiofilm activity of Artocarpus lakoocha extract against various Candida. Anticandidal activity of A. lakoocha extract was determined using an agar well diffusion method. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were assessed using a method of the Clinical and Laboratory Standards Institute. A time kill assay was also performed. Antibiofilm activity was investigated using a 3-[4, 5- dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide (MTT) assay. The extract was found to be effective against all tested Candida strains with MIC ranging from 0.05 to 3.12 mg/ml and MFC ranging from 0.10 to 25 mg/ml. The killing activity depended on the time and concentrations of the extract. A. lakoocha extract acts as a potent antibiofilm agent with dual actions, preventing and eradicating the biofilm. Results suggest that A. lakoocha extract is a potential source of natural anticandidal agent, which may be useful for prevention or treatment of candidiasis.

Keywords

• Artocarpus lakoocha
• Candida
• anticandidal activity
• antibiofilm activity