Journal of King Saud University: Science (Jul 2022)
Stroke preventing effect of resveratrol isolated from fungi and in vivo activity in male albino rats
Abstract
The main objective of the study was to analyze the stroke preventing property of resveratrol from Alternaria alternata SK. The endophytic fungus A. alternata SK was isolated from the Brinjal plant. The isolated fungus was characterized based on morphological characters and molecular methods. It was cultured in submerged cultivation and resveratrol was isolated. The isolated resveratrol was used to test stroke preventing properties using animal model. The experimental male Albino rat fed with resveratrol decreased HDL cholesterol level in serum. The amount of malondialdehyde significantly lower in the cardiac tissues of Albino AD rat treated with resveratrol (P < 0.05). Likewise, the level of malondialdehyde in the haemolysate sample showed significant variation than control animal (P < 0.05). Administered resveratrol improved antioxidant potential. The amount of antioxidant enzymes such as, SOD, CAT and GPx significantly higher in resveratrol administered Albino rats (P < 0.05) than control rat. The amount of GSH, α-tocopherol and ascorbate in cardiac muscle of AD rats fed with resveratrol increased significantly than in AD Albino rats (P < 0.05). The amount of aspartate amino transferase, alanine amino transferase, alkaline phosphatase, and lactate dehydrogenate were reduced in experimental rats treated with resveratrol than control (P < 0.05). The experimental group treated with resveratrol decreased the level of LDH (1.63 ± 0.128 nmol NADPH/mg) than ischemia group (2.08 ± 0.11 nmol NADPH/mg) (P < 0.05). The amount of H2O2 was found to be high in ischemia group (428.2 ± 2.9 nmol) and it reduced (320.3 ± 10.2 nmol) in resveratrol treated animal (P < 0.05). In vivo analysis of cerebral ischemia/reperfusion in Albino rats revealed the protective role of resveratrol on LDH and H2O2 in hippocampus region.
