Inhibition of Peroxidation Potential and Protein Oxidative Damage by Metal Mangiferin Complexes

Abstract

Background: Metal coordination complexes of polyphenolic compounds have been claimed to have better antioxidant and protection against protein oxidative damage effects than the isolated ligands. Whereas flavonoids have been extensively studied, xanthones such as mangiferin are lacking extensive research. Methods: Cu (II), Zn (II), and Se (IV) mangiferin complexes were synthesized with different stoichiometric ratios. Products were isolated by preparative chromatography and subjected to spectral analysis by FT-IR, HPLC-DAD, and HPLC-ESI-MS. The inhibition effects on peroxidation potential and protein oxidative damage were determined for all the metal–MF complexes. Results: Eight metal–MF complexes were isolated. Cu (II)–MF complexes did not improve MF antioxidant/protective effects; Zn (II) complexes (stoichiometric ratio 1:2) antioxidant/protective effects had no significant differences to MF; Zn (II)– and Se (IV)–MF complexes (stoichiometric ratio 1:3) showed the best inhibition effects on peroxidation potential (49.06% and 32.08%, respectively), and on the protection against protein oxidative damage (14.49% and 20.81%, respectively). Conclusions: The antioxidant/protective effects of Se (IV)– and Zn (II)–MF coordination complexes were significantly improved as compared to isolated MF, when the reaction between the metal salt and MF was performed with a stoichiometric ratio 1:3.

Keywords

• oxidative stress
• protein oxidative damage
• mangiferin
• metal–mangiferin complexes
• antioxidant therapy