Communications Biology (Mar 2023)

Targeting Ras-binding domain of ELMO1 by computational nanobody design

  • Chunlai Tam,
  • Mutsuko Kukimoto-Niino,
  • Yukako Miyata-Yabuki,
  • Kengo Tsuda,
  • Chiemi Mishima-Tsumagari,
  • Kentaro Ihara,
  • Mio Inoue,
  • Mayumi Yonemochi,
  • Kazuharu Hanada,
  • Takehisa Matsumoto,
  • Mikako Shirouzu,
  • Kam Y. J. Zhang

DOI
https://doi.org/10.1038/s42003-023-04657-w
Journal volume & issue
Vol. 6, no. 1
pp. 1 – 11

Abstract

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Structural analysis of the Ras-binding domain (RBD) of ELMO1 alone and in complex with the activator RhoG enables rational engineering and validation of a nanobody targeting ELMO1 RBD.