Using the design experiment RSM for the preparation of nanoparticles containing pyrazinamide, ethambutol and rifampin

Abstract

In this study, three-component nanoparticles of iron, nickel and lithium were synthesized with two methods of combined technique (co-precipitation, hydrothermal) and pechini sol-gel. The optimum conditions for producing magnetic nanoparticles (MNPs) by co-precipitation method were found to be at pH=13 with constant stirring rate of 1000 rpm and at temperature of 72 ˚C under nitrogen atmosphere. The situation for preparing nanoparticles by pechini sol-gel method including dissolving nitrate salt of three metals in water then adding citric acid and ethylene glycol respectively in temperatures 60 and 80 0 C with pH=7 and placing sample in temperatures 190 and 500 0 C for 720 and 60 minutes respectively. In the comparison of two methods, morphology and size of produced magnetic nanoparticles with combined method of co-precipitation- hydrothermal was more suitable. Response surface methodology was used to verify the best synthesized nanoparticles in size. The surface of MNPs was modified with silica and amine groups to develop a suitable drug delivery system.

Keywords

• magnetic nanoparticles
• response surface methodology
• pechini sol-gel
• hydrothermal