Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM

Jessica Tittelmeier; Silke Druffel-Augustin; Ania Alik; Ronald Melki; Carmen Nussbaum-Krammer

doi:10.1038/s42003-022-04289-6

Communications Biology (Dec 2022)

Dissecting aggregation and seeding dynamics of α-Syn polymorphs using the phasor approach to FLIM

Jessica Tittelmeier,
Silke Druffel-Augustin,
Ania Alik,
Ronald Melki,
Carmen Nussbaum-Krammer

Affiliations

Jessica Tittelmeier: Center for Molecular Biology of Heidelberg University (ZMBH) and German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance
Silke Druffel-Augustin: Center for Molecular Biology of Heidelberg University (ZMBH) and German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance
Ania Alik: Institute Francois Jacob (MIRCen), CEA, and Laboratory of Neurodegenerative Diseases, CNRS
Ronald Melki: Institute Francois Jacob (MIRCen), CEA, and Laboratory of Neurodegenerative Diseases, CNRS
Carmen Nussbaum-Krammer: Center for Molecular Biology of Heidelberg University (ZMBH) and German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance

DOI: https://doi.org/10.1038/s42003-022-04289-6
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 13

Abstract

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Fluorescence lifetime imaging microscopy combined with phasor analysis resolves seeding and aggregation dynamics of different α-synuclein polymorphs in a biosensor cell line.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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