Metabolites (Oct 2021)

Analytical Methodology for a Metabolome Atlas of Goat’s Plasma, Milk and Feces Using <sup>1</sup>H-NMR and UHPLC-HRMS

  • Cécile Martias,
  • Julie Gatien,
  • Léa Roch,
  • Nadine Baroukh,
  • Sylvie Mavel,
  • Antoine Lefèvre,
  • Frédéric Montigny,
  • Laurent Schibler,
  • Patrick Emond,
  • Lydie Nadal-Desbarats

DOI
https://doi.org/10.3390/metabo11100681
Journal volume & issue
Vol. 11, no. 10
p. 681

Abstract

Read online

Metabolomics has been increasingly used in animal and food sciences. Animal health is one of the most important factor that can also alter animal integrity and welfare. Some studies have already investigated the link between health and metabolic profile of dairy animals. These studies in metabolomics often consider a single type of sample using a single analytical platform (nuclear magnetic resonance or mass spectrometry). Only few studies with multi-platform approaches are also used with a single or a multi type of sample, but they mainly consider dairy cows’ metabolome although dairy goats present similar diseases, that it could be interesting to detect early to preserve animal health and milk production. This study aims to create a metabolic atlas of goat plasma, milk and feces, based on healthy animals. Our study describes a standard operating procedure for three goat matrices: blood plasma, milk, and feces using multiple platforms (NMR (1H), UHPLC (RP)-MS and UHPLC (HILIC)-MS) that follows a unique sample preparation procedure for each sample type to be analyzed on multi-platforms basis. Our method was evaluated for its robustness and allowed a better characterization of goat metabolic profile in healthy conditions.

Keywords