Cancer Management and Research (Aug 2021)
Diagnostic Value of Serum lncRNA HOTAIR Combined with Galectin-3 in Benign and Papillary Thyroid Carcinoma
Abstract
Li Li,1,* Jianjun Wang,1,* Zhongquan Li,2 Shuang Qiu,1 Junyu Cao,1 Yuan Zhao,1 Zhenfan Huang,1 Jie He,1 Feipeng Luo,3 Kunxian Yang1 1Department of Breast and Thyroid Surgery, The First People’s Hospital of Yunnan Province (Kunhua Hospital Affiliated to Kunming University of Science and Technology), Kunming, Yunnan, 650032, People’s Republic of China; 2Department of Anesthesiology, Yunnan General Hospital of Armed Police, Kunming, Yunnan, 650032, People’s Republic of China; 3Department of Cardiothoracic Surgery, The Second People’s Hospital of Baoshan City, Kunming, Yunnan, 650032, People’s Republic of China*These authors contributed equally to this workCorrespondence: Kunxian YangDepartment of Breast and Thyroid Surgery, The First People’s Hospital of Yunnan Province (Kunhua Hospital Affiliated to Kunming University of Science and Technology), No. 157, Jinbi Road, Xishan District, Kunming, Yunnan, 650032, People’s Republic of ChinaTel +86-0871-63627184Email [email protected]: Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer. LncRNA HOTAIR (HOx Transcript AntIsense RNA) and Galectin-3 are involved in PTC. This study explored the clinical effect of lncRNA HOTAIR/Galectin-3 on PTC patients.Methods: Subjects were assigned into PTC (160 cases) and benign thyroid tumor groups (150 cases). Fasting peripheral venous blood was collected. LncRNA HOTAIR/Galectin-3 expressions in serum were detected. Subjects were assigned into HOTAIR/Glactin-3 high/low expression groups and their correlation with age, gender, BMI, tumor size, pathological stage, TSH, TPO-Ab, and TG-Ab in PTC was analyzed. Receiver operating characteristic (ROC) curve was conducted on diagnostic efficacy of HOTAIR or/and Galectin-3. The difference of area under the curve (AUC) was compared and analyzed.Results: HOTAIR and Glactin-3 were higher in PTC group and correlated with tumor pathological stage. Higher HOTAIR/Glactin-3 expression indicated a more advanced TNM stage. LncRNA HOTAIR was positively correlated with TPO-Ab and TG-Ab. AUC of HOTAIR for PTC diagnosis was 0.895, with 96.00% specificity and 80.63% sensitivity. AUC of Glactin-3 for PTC diagnosis was 0.817, with 66.67% specificity and 78.75% sensitivity. AUC of HOTAIR combining with Glactin-3 for PTC diagnosis was 0.969 with 96.00% specificity and 87.50% sensitivity. AUC of lncRNA HOTAIR was higher than that of Glactin-3, while AUC of the combination was higher than that of lncRNA HOTAIR or Glactin-3.Conclusion: LncRNA HOTAIR and Glactin-3 were highly expressed in PTC. The combination detection of lncRNA HOTAIR/Glactin-3 had higher diagnostic efficiency on the differential diagnosis of benign thyroid tumor and PTC.Keywords: papillary thyroid carcinoma, LncRNA HOTAIR, Glactin-3, combination detection, benign thyroid tumor, ROC curve analysis, diagnostic efficiency, TPO-Ab, PTC
