Sublethal Exposure Effects of the Neonicotinoid Clothianidin Strongly Modify the Brain Transcriptome and Proteome in the Male Moth <i>Agrotis ipsilon</i>
Camille Meslin,
Françoise Bozzolan,
Virginie Braman,
Solenne Chardonnet,
Cédric Pionneau,
Marie-Christine François,
Dany Severac,
Christophe Gadenne,
Sylvia Anton,
Martine Maibèche,
Emmanuelle Jacquin-Joly,
David Siaussat
Affiliations
Camille Meslin
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
Françoise Bozzolan
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
Virginie Braman
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
Solenne Chardonnet
Plateforme Post-Génomique de la Pitié-Salpêtrière (P3S), UMS 37 PASS, INSERM, Sorbonne Université, 75013 Paris, France
Cédric Pionneau
Plateforme Post-Génomique de la Pitié-Salpêtrière (P3S), UMS 37 PASS, INSERM, Sorbonne Université, 75013 Paris, France
Marie-Christine François
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
Dany Severac
MGX, BioCampus Montpellier, CNRS, INSERM, Université de Montpellier, 34000 Montpellier, France
Christophe Gadenne
Institut de Génétique Environnement et Protection des Plantes IGEPP, INRAE, Institut Agro, Université de Rennes, 49045 Angers, France
Sylvia Anton
Institut de Génétique Environnement et Protection des Plantes IGEPP, INRAE, Institut Agro, Université de Rennes, 49045 Angers, France
Martine Maibèche
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
Emmanuelle Jacquin-Joly
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
David Siaussat
Département Ecologie Sensorielle, Institut d’Ecologie et des Sciences de l’Environnement de Paris (iEES-Paris), Sorbonne Université, INRAE, CNRS, IRD, UPEC, Université de Paris, 75005 Paris, France
Insect pest management relies mainly on neurotoxic insecticides, including neonicotinoids such as clothianidin. The residual accumulation of low concentrations of these insecticides can have positive effects on target pest insects by enhancing various life traits. Because pest insects often rely on sex pheromones for reproduction and olfactory synaptic transmission is cholinergic, neonicotinoid residues could indeed modify chemical communication. We recently showed that treatments with low doses of clothianidin could induce hormetic effects on behavioral and neuronal sex pheromone responses in the male moth, Agrotis ipsilon. In this study, we used high-throughput RNAseq and proteomic analyses from brains of A. ipsilon males that were intoxicated with a low dose of clothianidin to investigate the molecular mechanisms leading to the observed hormetic effect. Our results showed that clothianidin induced significant changes in transcript levels and protein quantity in the brain of treated moths: 1229 genes and 49 proteins were differentially expressed upon clothianidin exposure. In particular, our analyses highlighted a regulation in numerous enzymes as a possible detoxification response to the insecticide and also numerous changes in neuronal processes, which could act as a form of acclimatization to the insecticide-contaminated environment, both leading to enhanced neuronal and behavioral responses to sex pheromone.
