Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography

Xin-Ying Li; Mei Yang; Jie-Yun Huang; Xiao-Xue Yu; Min-Qian Zhao; Zhi-Kun Liang; Zhi-Sheng Xie; Xin-Jun Xu

Journal of Pharmaceutical Analysis (Dec 2013)

Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography

Xin-Ying Li,
Mei Yang,
Jie-Yun Huang,
Xiao-Xue Yu,
Min-Qian Zhao,
Zhi-Kun Liang,
Zhi-Sheng Xie,
Xin-Jun Xu

Affiliations

Xin-Ying Li: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Mei Yang: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Jie-Yun Huang: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Xiao-Xue Yu: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Min-Qian Zhao: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Zhi-Kun Liang: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Zhi-Sheng Xie: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China
Xin-Jun Xu: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China; Corresponding author at: School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China. Tel: +86 13924061779, +86 20 39943041.

Journal volume & issue: Vol. 3, no. 6
pp. 429 – 433

Abstract

Read online

A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR. This method was simple, fast, convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus. Keywords: Schisandrae Sphenantherae Fructus, High-speed counter-current chromatography (HSCCC), Deoxyschizandrin

Published in Journal of Pharmaceutical Analysis

ISSN: 2095-1779 (Print); 2214-0883 (Online)
Publisher: Elsevier
Country of publisher: China
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmaceutical-analysis/

About the journal