Research of T-SOD and MDA in rabbit lens at the early period after vitrectomy combined with nonexpansile perfluoropropane gas

Abstract

AIM:To study the relationship between oxidative damage and the early pathological changes in rabbit lens after vitrectomy combined with nonexpansile perfluoropropane gas, by detecting T-SOD activity and MDA content at different stages after the operation. METHODS:Fourty five healthy new zealand rabbits were divided randomly to three groups:normal group, control group and experimental group. The control group was just performed vitrectomy with BSS; the experimental group was performed vitrectomy combined with nonexpansile perfluoropropane gas. The lens were made into tissue homogenates to detect T-SOD activity and MDA content by using spectrophotometry on the 1st,3th,8th,35th and 45th day after the operation. RESULTS:T-SOD activity: that in normal group was the hightest, that in experimental group was the lowest, and there was significant difference(PPCONCLUSION:Oxidative damage mechanism may be involved in the early damge of rabbit lens after vitrectomy combined with nonexpansile perfluoropropane gas.

Keywords

• vitrectomy
• perfluoropropane gas
• cataract
• oxidative damage