Delving into the Mechanisms of Sponge-Associated <i>Enterobacter</i> against Staphylococcal Biofilms
Anna Luiza Bauer Canellas,
Bruno Francesco Rodrigues de Oliveira,
Suzanne de Oliveira Nunes,
Camila Adão Malafaia,
Ana Claudia F. Amaral,
Daniel Luiz Reis Simas,
Ivana Correa Ramos Leal,
Marinella Silva Laport
Affiliations
Anna Luiza Bauer Canellas
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil
Bruno Francesco Rodrigues de Oliveira
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil
Suzanne de Oliveira Nunes
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil
Camila Adão Malafaia
Laboratório de Produtos Naturais e Ensaios Biológicos, DPNA, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Ana Claudia F. Amaral
Laboratório de Plantas Medicinais e Derivados, Farmanguinhos, Fiocruz, Rio de Janeiro 21041-250, Brazil
Daniel Luiz Reis Simas
Laboratório de Produtos Naturais e Ensaios Biológicos, DPNA, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Ivana Correa Ramos Leal
Laboratório de Produtos Naturais e Ensaios Biológicos, DPNA, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil
Marinella Silva Laport
Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-590, Brazil
Staphylococci are one of the most common causes of biofilm-related infections. Such infections are hard to treat with conventional antimicrobials, which often lead to bacterial resistance, thus being associated with higher mortality rates while imposing a heavy economic burden on the healthcare system. Investigating antibiofilm strategies is an area of interest in the fight against biofilm-associated infections. Previously, a cell-free supernatant from marine-sponge-associated Enterobacter sp. inhibited staphylococcal biofilm formation and dissociated the mature biofilm. This study aimed to identify the chemical components responsible for the antibiofilm activity of Enterobacter sp. Scanning electron microscopy confirmed that the aqueous extract at the concentration of 32 μg/mL could dissociate the mature biofilm. Liquid chromatography coupled with high-resolution mass spectrometry revealed seven potential compounds in the aqueous extract, including alkaloids, macrolides, steroids, and triterpenes. This study also suggests a possible mode of action on staphylococcal biofilms and supports the potential of sponge-derived Enterobacter as a source of antibiofilm compounds.
