Molecular identification of <it>Coccidioides </it>spp. in soil samples from Brazil

BMC Microbiology. 2011;11(1):108 DOI 10.1186/1471-2180-11-108


Journal Homepage

Journal Title: BMC Microbiology

ISSN: 1471-2180 (Online)

Publisher: BMC

LCC Subject Category: Science: Microbiology

Country of publisher: United Kingdom

Language of fulltext: English

Full-text formats available: PDF, HTML



Filho Antônio D

Eulálio Kelsen D

Cavalcante Maria AS

da Mota Fabio F

Rosado Alexandre S

de Macêdo Regina CL

Martins Liline MS

Lazéra Márcia S

Wanke Bodo


Blind peer review

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Time From Submission to Publication: 18 weeks


Abstract | Full Text

<p>Abstract</p> <p>Background</p> <p>Since 1991 several outbreaks of acute coccidioidomycosis (CM) were diagnosed in the semi-arid Northeast of Brazil, mainly related to disturbance of armadillo burrows caused by hunters while digging them for the capture of these animals. This activity causes dust contaminated with arthroconidia of <it>Coccidioides posadasii</it>, which, once inhaled, cause the mycosis. We report on the identification of <it>C. posadasii </it>in soil samples related to outbreaks of CM.</p> <p>Results</p> <p>Twenty four soil samples had their DNA extracted and subsequently submitted to a semi-nested PCR technique using specific primers. While only 6 (25%) soil samples were positive for <it>C. posadasii </it>by mice inoculation, all (100%) were positive by the molecular tool.</p> <p>Conclusion</p> <p>This methodology represents a simple, sensitive and specific molecular technique to determine the environmental distribution of <it>Coccidioides </it>spp. in endemic areas, but cannot distinguish the species. Moreover, it may be useful to identify culture isolates. Key-words: 1. Coccidioidomycosis. 2. <it>Coccidioides </it>spp. 3. <it>C. posadasii</it>. 4. Semi-arid. 5. Semi-nested PCR</p>