Data concerning the chromatographic isolation of bovine IgG from milk- and colostral whey
Hans-Jürgen Heidebrecht,
Bernadette Kainz,
Roland Schopf,
Klaus Godl,
Züleyha Karcier,
Ulrich Kulozik,
Beatrix Förster
Affiliations
Hans-Jürgen Heidebrecht
Chair of Food and Bioprocess Engineering, Technical University of Munich, Germany; ZIEL Institute for Food & Health, Technical University of Munich, Germany; Corresponding author at: Chair of Food and Bioprocess Engineering, Technical University of Munich, Weihenstephaner Berg 1, 85354 Freising, Germany.
Bernadette Kainz
Chair of Food and Bioprocess Engineering, Technical University of Munich, Germany
Roland Schopf
Chair of Food and Bioprocess Engineering, Technical University of Munich, Germany
Klaus Godl
Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilian-University, Munich, Germany
Züleyha Karcier
Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilian-University, Munich, Germany
Ulrich Kulozik
Chair of Food and Bioprocess Engineering, Technical University of Munich, Germany; ZIEL Institute for Food & Health, Technical University of Munich, Germany
Beatrix Förster
Molecular Animal Breeding and Biotechnology, Gene Center, Ludwig-Maximilian-University, Munich, Germany; Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands
Data included are related to the research article “Isolation of biofunctional bovine immunoglobulin G from milk- and colostral whey with mixed-mode chromatography at lab and pilot scale” (Heidebrecht et al., 2018) [1]. Data show individual bovine whey proteins in flow-through and elution fractions using different chromatographic resins as well as different binding and elution conditions. The relevant analytical methods for individual protein detection were SDS-PAGE and reversed phase- high performance liquid chromatography. The focus of the data is on the two mixed mode materials MEP HyperCel™ and Capto™-multimodal chromatography. Resins were used individually, in series and at different scale. Data provide information at which binding and elution conditions it is possible to isolate bovine IgG from milk and colostral whey and at which purity.
