Screening of New Microsatellite DNA Markers from the Genome of <em>Platyeriocheir formosa</em>

Abstract

The catadromous <em>Platyeriocheir formosa</em> is a crab endemic in Taiwan. To conserve <em>P. formosa </em>population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote the study of its population genetics in the future. In this study, more than 70 microsatellite sequences were found. Among these, 18 loci were selected to analyze the genetic diversity of <em>P. formosa</em>. With the exception of the Pfo15 locus, all of the remaining loci were polymorphic with allelic numbers ranging from 3–14. Heterozygosity within all 17 polymorphic loci ranged from 0.2–0.95 with an average of 0.55, which suggested that these loci are proper markers for studying population genetics. After we tested cross-specific amplification, eight and six primer sets could be successfully used for the amplification of microsatellite loci in morphologically similar <em>Eriocheir</em> <em>sinensis</em> and <em>E. japonica</em>, respectively; this suggests that they are useful markers for closely related species.

Keywords

• catadromous
• crab
• cross-specific amplification
• polymorphic loci