Effects of environmentally relevant concentrations of micro(nano)plastics on aquatic microorganisms: Changes in potential function but not in overall composition

Abstract

Micro(nano)plastics (MNPs) are a growing problem as persistent environmental pollutants. Here, we investigated the impact of MNPs on microorganisms in aquatic microbial floc exposed to NPs (80 ​nm) and MPs (8 ​μm) for 35 days. Water quality indicators were tested weekly and microbiological analyses were conducted on Day 7 and 28 after exposure. The results showed that there were significant differences in the levels of total ammonia nitrogen or nitrite between the MNPs groups and the control group, spanning from Day 7 to Day 28. For the microbial response, microbial community richness in the NPs and MPs groups were significantly increased at Day 7. Functional prediction showed that the relative abundances of bacteria associated with the “Forms Biofilms”, “Potentially Pathogenic”, “Plastic Degradation” and nitrogen cycle processes were significantly different after MNPs exposure. The results suggest that MNPs had no significant effect on the microbial diversity of mature microbial flocs. Findings suggest MPs could cause an increase in the relative abundance of potentially pathogenic bacteria, while NPs do not. In addition, stress associated with MNPs affected the nitrogen cycle of microorganisms, and NPs exerted greater impacts than MPs. Findings from this study further our understanding of the impact of MNPs at environmentally relevant concentrations on microorganisms in aquatic ecosystems.

Keywords

• Nano-plastics
• Micro-plastics
• Aquatic microorganism
• Pathogenic bacteria
• Nitrogen cycle