Development of an Immunochromatography Assay to Detect Marburg Virus and Ravn Virus
Katendi Changula,
Masahiro Kajihara,
Shino Muramatsu,
Koji Hiraoka,
Toru Yamaguchi,
Yoko Yago,
Daisuke Kato,
Hiroko Miyamoto,
Akina Mori-Kajihara,
Asako Shigeno,
Reiko Yoshida,
Corey W. Henderson,
Andrea Marzi,
Ayato Takada
Affiliations
Katendi Changula
Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia
Masahiro Kajihara
Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
Shino Muramatsu
DENKA Co., Ltd., Tokyo 103-8338, Japan
Koji Hiraoka
DENKA Co., Ltd., Tokyo 103-8338, Japan
Toru Yamaguchi
DENKA Co., Ltd., Tokyo 103-8338, Japan
Yoko Yago
DENKA Co., Ltd., Tokyo 103-8338, Japan
Daisuke Kato
DENKA Co., Ltd., Tokyo 103-8338, Japan
Hiroko Miyamoto
Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
Akina Mori-Kajihara
Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
Asako Shigeno
Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
Reiko Yoshida
Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
Corey W. Henderson
Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
Andrea Marzi
Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA
Ayato Takada
Division of Global Epidemiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan
The recent outbreaks of Marburg virus disease (MVD) in Guinea, Ghana, Equatorial Guinea, and Tanzania, none of which had reported previous outbreaks, imply increasing risks of spillover of the causative viruses, Marburg virus (MARV) and Ravn virus (RAVV), from their natural host animals. These outbreaks have emphasized the need for the development of rapid diagnostic tests for this disease. Using monoclonal antibodies specific to the viral nucleoprotein, we developed an immunochromatography (IC) assay for the rapid diagnosis of MVD. The IC assay was found to be capable of detecting approximately 102−4 50% tissue culture infectious dose (TCID50)/test of MARV and RAVV in the infected culture supernatants. We further confirmed that the IC assay could detect the MARV and RAVV antigens in the serum samples from experimentally infected nonhuman primates. These results indicate that the IC assay to detect MARV can be a useful tool for the rapid point-of-care diagnosis of MVD.
