Level of sialylation and galactosylation of blood plasma glycoproteins under the influence of alkylating chemotherapy in patients with chronic lymphocytic leukemia

Abstract

Level of sialylation, galactosylation of α1-acid glycoprotein and plasma fibronectin in patients with chronic lymphocytic leukemia before the alkylating therapy and at its different stages were studied. Treatment of patients with chronic lymphocytic leukemia destined chemotherapeutic regimens according to the program СOP (cyclophosphamide, onkovin and prednisolone). Lectin-enzyme analyses with sialospecific Maackia amurensis lectin MAA-II, sambucus nigra lectin SNA and galactospecific ricinus communis lectin were used. Significant decrease of sialylation and galactosylation of both studied proteins in chronic lymphocytic leukemia and dynamic changes of these parameters during the first day of treatment and in two months were shown. Alkylating chemotherapy of chronic lymphocytic leukemia patients influences as the level of sialylation as the level of galactosylation both studied glycoproteins. Restoration of galactose within the N-glycans of α1-acid glycoprotein and fibronectin was observed after chemotherapy. Obtained data could be useful for the better understanding of the mechanisms of action of selected treatment and defense reactions of the organism.

Keywords

• fibronectin
• α1-acid glycoprotein
• sialic acid
• galactose
• chronic lymphocytic leukemia
• cop-therapy