A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli

Abstract

Key points 1. Combining several parameters suggested in different papers in one experiment to get the active enzyme. 2. Decreasing the temperature of induction and the amount of inducer and using a chaperone-like tag simultaneously to help the protein folding system of the bacteria. 3. Using a statistical method (RSM) to analyze a number of treatments and prediction of the most effective ones to optimize the expression system.

Keywords

• Recombinant enterokinase
• Escherichia coli
• Response surface methodology
• Protein folding
• Affinity chromatography