The in vitro germplasm conservation of mangosteen (Garcinia mangostana L.) and longkong (Lansium domesticum Corr.) was investigated by suppression of shoot growth using growth retardants and increasing the osmotic potential of culture medium. Abscisic acid (ABA) at 2 mg/l affected to suppress shoot growth of mangosteen and longkong more than paclobutrazol. Although shoot growth of both plants was consequently inhibited for 12 months of conservation, the regrowth ability was successfully obtained when transferred to proliferation medium. Besides, increasing the concentration of agar up to 14 g/l was found to be more effective to suppress shoot growth of mangosteen and longkong by treatment of tissue desiccation. Severe succulence of mangosteen shoot was observed when shoots were cultured in a medium containing mannitol.