Data in Brief (Feb 2023)

Dataset from de novo transcriptome assembly of Myristica fatua leaves using MinION nanopore sequencer

  • Deden Derajat Matra,
  • M Adrian,
  • Karmanah,
  • Jakty Kusuma,
  • Jérôme Duminil,
  • Sobir,
  • Roedhy Poerwanto

Journal volume & issue
Vol. 46
p. 108838

Abstract

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Myristica fatua is a tropical fruit tree species originating from Indonesia. Very few genomic resources are available for the species. We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). The assembled full-length transcript was constructed using the RATTLE program and assembled 21,098 transcripts. The transcript ranged from 201 – 14,174 bp, and N50 was 2,017 bp. The transcripts were annotated with the UNIPROT database using BlastX. The functional annotation was performed using Blast2go software. The 8,445 microsatellite motif-containing contigs were identified. The raw reads are deposited in the ENA (European Nucleotide Archive) with ENA experiment accession number ERX6798613.

Keywords