Frontiers in Cellular and Infection Microbiology (Jun 2018)
Cas1 and Cas2 From the Type II-C CRISPR-Cas System of Riemerella anatipestifer Are Required for Spacer Acquisition
- Yang He,
- Yang He,
- Yang He,
- Mingshu Wang,
- Mingshu Wang,
- Mingshu Wang,
- Mafeng Liu,
- Mafeng Liu,
- Mafeng Liu,
- Li Huang,
- Li Huang,
- Li Huang,
- Chaoyue Liu,
- Chaoyue Liu,
- Chaoyue Liu,
- Xin Zhang,
- Xin Zhang,
- Xin Zhang,
- Haibo Yi,
- Haibo Yi,
- Haibo Yi,
- Anchun Cheng,
- Anchun Cheng,
- Anchun Cheng,
- Dekang Zhu,
- Dekang Zhu,
- Qiao Yang,
- Qiao Yang,
- Qiao Yang,
- Ying Wu,
- Ying Wu,
- Ying Wu,
- Xinxin Zhao,
- Xinxin Zhao,
- Xinxin Zhao,
- Shun Chen,
- Shun Chen,
- Shun Chen,
- Renyong Jia,
- Renyong Jia,
- Renyong Jia,
- Shaqiu Zhang,
- Shaqiu Zhang,
- Shaqiu Zhang,
- Yunya Liu,
- Yunya Liu,
- Yunya Liu,
- Yanling Yu,
- Yanling Yu,
- Yanling Yu,
- Ling Zhang,
- Ling Zhang,
- Ling Zhang
Affiliations
- Yang He
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Yang He
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Yang He
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mingshu Wang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mingshu Wang
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Mingshu Wang
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mafeng Liu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Mafeng Liu
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Mafeng Liu
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Li Huang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Li Huang
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Li Huang
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Chaoyue Liu
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Chaoyue Liu
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Chaoyue Liu
- Department of Microbiology and Immunology, North Sichuan Medical College, Nanchong, China
- Xin Zhang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xin Zhang
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Xin Zhang
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Haibo Yi
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Haibo Yi
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Haibo Yi
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Anchun Cheng
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Anchun Cheng
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Anchun Cheng
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Dekang Zhu
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Dekang Zhu
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Qiao Yang
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Qiao Yang
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ying Wu
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Ying Wu
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xinxin Zhao
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Xinxin Zhao
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Xinxin Zhao
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shun Chen
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Shun Chen
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Renyong Jia
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Renyong Jia
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Renyong Jia
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shaqiu Zhang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Shaqiu Zhang
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Shaqiu Zhang
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Yunya Liu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Yunya Liu
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Yunya Liu
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Yanling Yu
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Yanling Yu
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Yanling Yu
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ling Zhang
- Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- Ling Zhang
- Key Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China
- Ling Zhang
- Avian Diseases Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fcimb.2018.00195
- Journal volume & issue
-
Vol. 8
Abstract
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins provide acquired genetic immunity against the entry of mobile genetic elements (MGEs). The immune defense provided by various subtypes of the CRISPR-Cas system has been confirmed and is closely associated with the formation of immunological memory in CRISPR arrays, called CRISPR adaptation or spacer acquisition. However, whether type II-C CRISPR-Cas systems are also involved in spacer acquisition remains largely unknown. This study explores and provides some definitive evidence regarding spacer acquisition of the type II-C CRISPR-Cas system from Riemerella anatipestifer (RA) CH-2 (RA-CH-2). Firstly, introducing an exogenous plasmid into RA-CH-2 triggered spacer acquisition of RA CRISPR-Cas system, and the acquisition of new spacers led to plasmid instability in RA-CH-2. Furthermore, deletion of cas1 or cas2 of RA-CH-2 abrogated spacer acquisition and subsequently stabilized the exogenous plasmid, suggesting that both Cas1 and Cas2 are required for spacer acquisition of RA-CH-2 CRISPR-Cas system, consistent with the reported role of Cas1 and Cas2 in type I-E and II-A systems. Finally, assays for studying Cas1 nuclease activity and the interaction of Cas1 with Cas2 contributed to a better understanding of the adaptation mechanism of RA CRISPR-Cas system. This is the first experimental identification of the naïve adaptation of type II-C CRISPR-Cas system.
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