PLoS ONE (Jan 2012)

Inhibition of the Pim1 oncogene results in diminished visual function.

  • Jun Yin,
  • Lisa Shine,
  • Francis Raycroft,
  • Sudhakar Deeti,
  • Alison Reynolds,
  • Kristin M Ackerman,
  • Antonino Glaviano,
  • Sean O'Farrell,
  • Olivia O'Leary,
  • Claire Kilty,
  • Ciaran Kennedy,
  • Sarah McLoughlin,
  • Megan Rice,
  • Eileen Russell,
  • Desmond G Higgins,
  • David R Hyde,
  • Breandan N Kennedy

DOI
https://doi.org/10.1371/journal.pone.0052177
Journal volume & issue
Vol. 7, no. 12
p. e52177

Abstract

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Our objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis of transcriptomic data revealed Jak-Stat signalling as the pathway most enriched in the eye, as visual function develops. Real-time PCR, western blotting, immunohistochemistry and in situ hybridization data confirm that multiple Jak-Stat pathway genes are up-regulated in the zebrafish eye between 3-5 days post-fertilisation, times associated with significant maturation of vision. One of the most up-regulated Jak-Stat genes is the proto-oncogene Pim1 kinase, previously associated with haematological malignancies and cancer. Loss of function experiments using Pim1 morpholinos or Pim1 inhibitors result in significant diminishment of visual behaviour and function. In summary, we have identified that enhanced expression of Jak-Stat pathway genes correlates with maturation of visual function and that the Pim1 oncogene is required for normal visual function.