Employing Titanium Dioxide Nanoparticles as Biostimulant against Salinity: Improving Antioxidative Defense and Reactive Oxygen Species Balancing in Eggplant Seedlings

Abstract

Salinity is a major abiotic stress that affects the agricultural sector and poses a significant threat to sustainable crop production. Nanoparticles (NPs) act as biostimulants and significantly mitigate abiotic stress. In this context, this experiment was designed to assess the effects of foliar application of titanium dioxide (TiO2) nanoparticles at 200 and 400 ppm on the growth of eggplant (Solanum melongena) seedlings under moderate (75 mM) and high (150 mM) salinity stress. The TiO2-NPs employed were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and scanning electron microscopy (SEM) analysis. The seedlings were assessed physiologically, growth-wise, and biochemically. The seedlings were significantly affected by their physiological attributes (Fv′/Fm′, Fv/Fm, NPQ), growth (root length, shoot length, number of leaves, fresh biomass, dry biomass, leaf greenness), antioxidative enzymes (SOD, POD, CAT, APx, GR), stress indicators (H2O2, MDA), and toxic ion (Na+) concentrations. The maximum decrease in physiological and growth attributes in eggplant seedling leaves was observed with no TiO2-NP application at 150 mM NaCl. Applying TiO2-NPs at 200 ppm showed significantly less decrease in Fv’/Fm’, root length, shoot length, number of leaves, fresh biomass, dry biomass, and leaf greenness. In contrast, there were larger increases in SOD, POD, CAT, APx, GR, and TSP. This led to less accumulation of H2O2, MDA, and Na+. No significant difference was observed in higher concentrations of TiO2-NPs compared to the control. Therefore, TiO2-NPs at 200 ppm might be used to grow eggplant seedlings at moderate and high salinity.

Keywords

• antioxidative enzymes
• reactive oxygen species
• nanotechnology
• sodium toxicity
• vegetables