<i>Arthrobotrys musiformis</i> (Orbiliales) Kills <i>Haemonchus contortus</i> Infective Larvae (Trichostronylidae) through Its Predatory Activity and Its Fungal Culture Filtrates
Gustavo Pérez-Anzúrez,
Agustín Olmedo-Juárez,
Elke von-Son de Fernex,
Miguel Ángel Alonso-Díaz,
Edgar Jesús Delgado-Núñez,
María Eugenia López-Arellano,
Manasés González-Cortázar,
Alejandro Zamilpa,
Ana Yuridia Ocampo-Gutierrez,
Adolfo Paz-Silva,
Pedro Mendoza-de Gives
Affiliations
Gustavo Pérez-Anzúrez
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico
Agustín Olmedo-Juárez
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico
Elke von-Son de Fernex
Tropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre CP 93600, Mexico
Miguel Ángel Alonso-Díaz
Tropical Livestock Center, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Martínez de la Torre CP 93600, Mexico
Edgar Jesús Delgado-Núñez
Faculty of Agricultural, Livestock and Environmental Sciences, Autonomous University of the State of Guerrero, Iguala de la Independencia CP 40040, Mexico
María Eugenia López-Arellano
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico
Manasés González-Cortázar
South Biomedical Research Center, Social Security Mexican Institute (CIBIS-IMSS), Xochitepec CP 62790, Mexico
Alejandro Zamilpa
South Biomedical Research Center, Social Security Mexican Institute (CIBIS-IMSS), Xochitepec CP 62790, Mexico
Ana Yuridia Ocampo-Gutierrez
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico
Adolfo Paz-Silva
Department of Animal Pathology, Faculty of Veterinary, University of Santiago de Compostela, 27142 Lugo, Spain
Pedro Mendoza-de Gives
Laboratory of Helminthology, National Centre for Disciplinary Research in Animal Health and Innocuity (CENID-SAI), National Institute for Research in Forestry, Agriculture and Livestock, INIFAP-SADER, Morelos, Jiutepec CP 62550, Mexico
Haemonchus contortus (Hc) is a parasite affecting small ruminants worldwide. Arthrobotrys musiformis (Am) is a nematode-trapping fungi that captures, destroys and feeds on nematodes. This study assessed the predatory activity (PA) and nematocidal activity (NA) of liquid culture filtrates (LCF) of Am against Hc infective larvae (L3), and additionally, the mycochemical profile (MP) was performed. Fungal identification was achieved by traditional and molecular procedures. The PA of Am against HcL3 was performed in water agar plates. Means of non-predated larvae were recorded and compared with a control group without fungi. LCF/HcL3 interaction was performed using micro-tittering plates. Two media, Czapek–Dox broth (CDB) and sweet potato dextrose broth (SPDB) and three concentrations, were assessed. Lectures were performed after 48 h interaction. The means of alive and dead larvae were recorded and compared with proper negative controls. The PA assessment revealed 71.54% larval reduction (p p < 0.05). Alkaloids and saponins were identified in both media; meanwhile, coumarins were only identified in CDB. The NA was only found in CDB, but not in SPDB. Coumarins could be responsible for the NA.
