Functional Access to Neuron Subclasses in Rodent and Primate Forebrain
Preeti Mehta,
Lauren Kreeger,
Dennis C. Wylie,
Jagruti J. Pattadkal,
Tara Lusignan,
Matthew J. Davis,
Gergely F. Turi,
Wen-Ke Li,
Matthew P. Whitmire,
Yuzhi Chen,
Bridget L. Kajs,
Eyal Seidemann,
Nicholas J. Priebe,
Attila Losonczy,
Boris V. Zemelman
Affiliations
Preeti Mehta
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Lauren Kreeger
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Dennis C. Wylie
Center for Computational Biology and Bioinformatics, University of Texas, Austin, TX 78712, USA
Jagruti J. Pattadkal
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Tara Lusignan
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Matthew J. Davis
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Gergely F. Turi
Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA; Department of Neuroscience, Columbia University, New York, NY 10027, USA
Wen-Ke Li
Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA; Department of Neuroscience, Columbia University, New York, NY 10027, USA
Matthew P. Whitmire
Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Department of Psychology, University of Texas, Austin, TX 78712, USA
Yuzhi Chen
Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Department of Psychology, University of Texas, Austin, TX 78712, USA
Bridget L. Kajs
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Eyal Seidemann
Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Department of Psychology, University of Texas, Austin, TX 78712, USA
Nicholas J. Priebe
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Center for Perceptual Systems, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA
Attila Losonczy
Kavli Institute for Brain Science, Columbia University, New York, NY 10027, USA; Department of Neuroscience, Columbia University, New York, NY 10027, USA
Boris V. Zemelman
Center for Learning and Memory, University of Texas, Austin, TX 78712, USA; Department of Neuroscience, University of Texas, Austin, TX 78712, USA; Corresponding author
Summary: Viral vectors enable foreign proteins to be expressed in brains of non-genetic species, including non-human primates. However, viruses targeting specific neuron classes have proved elusive. Here we describe viral promoters and strategies for accessing GABAergic interneurons and their molecularly defined subsets in the rodent and primate. Using a set intersection approach, which relies on two co-active promoters, we can restrict heterologous protein expression to cortical and hippocampal somatostatin-positive and parvalbumin-positive interneurons. With an orthogonal set difference method, we can enrich for subclasses of neuropeptide-Y-positive GABAergic interneurons by effectively subtracting the expression pattern of one promoter from that of another. These methods harness the complexity of gene expression patterns in the brain and significantly expand the number of genetically tractable neuron classes across mammals. : Mehta et al. describe methods for accessing inhibitory neuron subclasses in the mammalian forebrain. Keywords: inhibitory neurons, somatostatin, parvalbumin, neuropeptide-Y, cell type-specific promoter, adeno-associated virus, rodent, marmoset, macaque, functional in vivo imaging
