The ٍeffect of Eight Weeks of Moderate and High Intensity Resistance Training on micro-RNAs Associated with Reverse Cholesterol Transport in Older Wistar rats

Abstract

Purpose: Aging is associated with systemic dysfunctions in lipid metabolism and chronic inflammatory state which contribute to atherosclerotic. The purpose of this study was to investigate the effect of moderate and high intensity resistance training on micro-RNAs Associated with reverse cholesterol transport in Wistar elderly rats.Methods: Thirty male Wistar rats (23 months old) were randomly, based on the weight of rats at the start of the study, divided into two experiment and one control group including moderate intensity resistance training (n = 10), high intensity resistance training (n = 10) and the control group (n = 10). Resistance training included 8 weeks of climbing a ladder with high intensity (80% MVCC) and moderate intensity (60% of MVCC) and 5 days a week. After completing training, expression of mir-33 and miR-144 and ABCA1 were measured RT-PCR technique. The statistical analysis was performed using ANOVA test with significance level of (P ≤0.05).Results: There was significance decrease in expression of miR-33a and miR-144 and increase in expression of ABCA1 in both high and moderate resistance groups (P ≤0.05). Also, there was significant difference between the mRNA expression of ABCA1 levels in high and moderate resistance training (P>0.05). However, there was no significant difference expression of miR-33a and miR-144 level between groups.Conclusion: It seems that moderate and high intensity resistance training can cause decrease the expression of miR-33a and miR-144 result to increase in mRNA expression of ABCA1.

Keywords

• 33a
• mir
• 144
• abca1
• atherosclerosis