Simultaneous determination of 6 flavonoids in health care products by high performance liquid chromatography

Abstract

Objective: To establish a method for simultaneous determination of wogonoside, 4'-hydroxywogonin, norwogonin, baicalein, wogonin and chrysin in health care products by high performance liquid chromatography （HPLC）. Methods: The samples were extracted with acetone, C18 solid phase extraction column was used for purification. The target compounds were determined by HPLC, quantified by matrix-matched standard curve method. Phenomenex Luna®C18（250 mm×4.6 mm, 5 μm）column was applied, mobile phase was acctonitrile-acetic acid, using gradient elution）at the flow rate of 1.0 mL/min. The temperature of column was 30 ℃ and the detection wavelength was set at 280 nm. Results: The results showed that these 6 target compounds had good separation and the corresponding concentration had a good linear relationship with the peak area. Correlation coefficients （R2） were all greater than 0.99, RSDs of precision and stability tests were within 3.08% and the average recoveries were 83%~100% （RSD＜4.63%）. The limits of detection were 0.02~0.07 mg/kg. The limits of quantification were 0.08~0.25 mg/kg. Conclusion: This method is suitable for simultaneous determination of flavonoids in health care products with good reproducibility, and less consumption of organic solvents.

Keywords

• high performance liquid chromatography
• flavonoids
• health care products