Correlation analysis between HPLC fingerprint of Smilax riparia and antioxidant activity

Abstract

In order to investigate the relationship between antioxidant efficacy and active components of Smilax riparia, the HPLC fingerprint of S. riparia extracts were determined, similarity evaluation and cluster analysis were carried out, while analysis software was used for PLSR to establish the spectra effect relationship, and validation test of in vitro anti-oxidation was carried out. The results were as follows: (1)HPLC fingerprints of 13 batches of S. riparia with 14 main common peaks were established. (2) Thirteen batches of S. riparia samples were grouped into two categories, the samples of S. riparia with close geographical location were clustered into one group, and the chemical fingerprints of S. riparia with close geographical location were highly similar. (3) PLSR showed that the areas of peaks 1, 2, 3, 5, 6, 9 and 14 in the fingerprint were positively correlated with the antioxidant effect, while the areas of peaks 4, 7, 8, 10, 11 and 12 were negatively correlated with the antioxidant effect, the VIP values of peaks 9, 11, 3, 4 and 5 were all greater than 1. (4) Peak 9 was oleanolic acid, Peak 10 was ursolic acid, and Peak 9 had the largest scavenging capacity for ABTS free radicals. Therefore, antioxidant efficacy of S. riparia is contributed by a combined action of multi-components rather than one component, and Peak 9 (oleanolic acid) may be the material basis of the antioxidation effect of S. riparia.

Keywords

• smilax riparia
• hplc
• fingerprint
• antioxidant
• spectrum-effect relationship