Magnoflorine—Isolation and the Anticancer Potential against NCI-H1299 Lung, MDA-MB-468 Breast, T98G Glioma, and TE671 Rhabdomyosarcoma Cancer Cells
Estera Okon,
Wirginia Kukula-Koch,
Marta Halasa,
Agata Jarzab,
Marzena Baran,
Magdalena Dmoszynska-Graniczka,
Apostolis Angelis,
Eleftherios Kalpoutzakis,
Malgorzata Guz,
Andrzej Stepulak,
Anna Wawruszak
Affiliations
Estera Okon
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Wirginia Kukula-Koch
Department of Pharmacognosy, Medical University of Lublin, 20-093 Lublin, Poland
Marta Halasa
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Agata Jarzab
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Marzena Baran
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Magdalena Dmoszynska-Graniczka
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Apostolis Angelis
Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimioupoli Zografou, 15771 Athens, Greece
Eleftherios Kalpoutzakis
Laboratory of Pharmacognosy and Natural Products Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Panepistimioupoli Zografou, 15771 Athens, Greece
Malgorzata Guz
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Andrzej Stepulak
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Anna Wawruszak
Department of Biochemistry and Molecular Biology, Medical University of Lublin, 20-093 Lublin, Poland
Magnoflorine (MGN) is a quaternary aporphine alkaloid that exhibits numerous therapeutic properties, including neuropsychopharmacological, anti-anxiety, immunomodulatory, anti-inflammatory, antioxidant, or antifungal activities. The aim of the present study was an investigation of the influence of MGN on viability, proliferation, induction of apoptosis, and cell cycle arrest in NCI-H1299 lung, MDA-MB-468 breast, T98G glioma, and TE671 rhabdomyosarcoma cancer cells. MGN was isolated from the roots of Berberis cretica L. by counter-current partition chromatography (CPC). Cell viability and proliferation assessments were performed by means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and 5-bromo-2ʹ-deoxyuridine (BrDU) assays, respectively. The induction of apoptosis and cell cycle progression was measured using fluorescence-activated cell sorting analysis. MGN in high doses inhibits proliferation, induces apoptosis, and inhibits cell cycle in S/G2 phases in a dose-dependent manner. MGN seems to be a promising anti-cancer compound in therapy of some types of lung, breast, glioma, and rhabdomyosarcoma cancers, for which current standard therapies are limited or have severe strong side effects.
