Nature Communications (Jan 2021)

Universal toxin-based selection for precise genome engineering in human cells

  • Songyuan Li,
  • Nina Akrap,
  • Silvia Cerboni,
  • Michelle J. Porritt,
  • Sandra Wimberger,
  • Anders Lundin,
  • Carl Möller,
  • Mike Firth,
  • Euan Gordon,
  • Bojana Lazovic,
  • Aleksandra Sieńska,
  • Luna Simona Pane,
  • Matthew A. Coelho,
  • Giovanni Ciotta,
  • Giovanni Pellegrini,
  • Marcella Sini,
  • Xiufeng Xu,
  • Suman Mitra,
  • Mohammad Bohlooly-Y,
  • Benjamin J. M. Taylor,
  • Grzegorz Sienski,
  • Marcello Maresca

DOI
https://doi.org/10.1038/s41467-020-20810-z
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 14

Abstract

Read online

Genome engineering in cell lines or human stem cells often has poor efficiency, limiting the development of research and therapeutic applications. Here, the authors use a toxin-based selection system for precise bi-allelic engineering in cells and in vivo.