High Retention and Purification of Bromelain Enzyme (<i>Ananas comosus</i> L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques
Felix M. Carbajal Gamarra,
José C. C. Santana,
Segundo A. V. Llanos,
Jorge A. Heredia Pérez,
Fábio Richard Flausino,
Ada P. B. Quispe,
Pedro Córdova Mendoza,
Rosangela M. Vanalle,
Carmen Carreño-Farfan,
Fernando T. Berssaneti,
Roberto R. de Souza,
Elias B. Tambourgi
Affiliations
Felix M. Carbajal Gamarra
Energy Engineering, University of Brasilia, FGA-UnB, St. Leste Projeção A—Gama Leste, Brasilia 72444-240, DF, Brazil
José C. C. Santana
Department of Management Engineering, Federal University of ABC, University Mall, São Bernardo do Campo 09606-045, SP, Brazil
Segundo A. V. Llanos
Facultad de Ingeniería Química e Industrias Alimentarias, CYMAIDS, Universidad Nacional Pedro Ruiz Gallo, Calle Juan XXIII 391, Lambayeque 14013, Peru
Jorge A. Heredia Pérez
Business School, Universidad del Pacífico, Calle Sanchez Cerro 2141, Jesús Maria, Lima 15072, Peru
Fábio Richard Flausino
Industrial Engineering Postgraduate Program, Nine July University, Vergueiro Street, Liberdade, São Paulo 01504-001, SP, Brazil
Ada P. B. Quispe
Facultad de Ingeniería Química e Industrias Alimentarias, CYMAIDS, Universidad Nacional Pedro Ruiz Gallo, Calle Juan XXIII 391, Lambayeque 14013, Peru
Pedro Córdova Mendoza
Facultad de Ingeniería Ambiental y Sanitaria, Universidad Nacional San Luis Gonzaga de Ica, Ciudad Universitaria, Km 305, Ica 11004, Peru
Rosangela M. Vanalle
Industrial Engineering Postgraduate Program, Nine July University, Vergueiro Street, Liberdade, São Paulo 01504-001, SP, Brazil
Carmen Carreño-Farfan
Facultad de Ciencias Biológicas, CYMAIDS, Universidad Nacional Pedro Ruiz Gallo, Calle Juan XXIII 391, Lambayeque 14013, Peru
Fernando T. Berssaneti
Department of Production Engineering, Polytechnic School of State University of São Paulo, Av. Prof. Luciano Gualberto, 1380—Butantã, São Paulo 05508-010, SP, Brazil
Roberto R. de Souza
Department of Chemical Engineering, Federal University of Sergipe, DEQ/UFS, University Campus “José Aloísio de Campos”, Av. Marechal Rondon, S/N, Rosa Elze, São Cristóvão 49100-000, SP, Brazil
Elias B. Tambourgi
School of Chemical Engineering, State University of Campinas, DESQ/FEQ/UNICAMP, University Campus “ZeferinoVaz”, Av. Albert Einstein, 500, Campinas 6066, São Paulo 13083-840, SP, Brazil
The demand for bromelian and pineapple fruit has been increasing substantially in the world because of their benefits for the human health and use in diverse areas. In this context, this work aimed to study the capacity of higher retention (concentration); bromelain activity underwent ultrafiltration from pineapple juice (Ananas comusus L. Merrill). All assays were carried out at pH 7.0 and 7.5, and at 0.05 and 0.40 bar of transmembrane pressures. Results have shown that at the best operating conditions, between 85 and 87% of bromelain activity was recovered using the plain membrane separation process at 0.05 bar. The ultrafiltration has shown the capacity to retain 100% of proteolytic activity of the bromelain extracted. The samples have kept the same physics properties after ultrafiltration, and the result was verified via electrophoresis. The bromelain enzyme obtained was characterized, and pH 7 and between 30 and 40 °C were the best conditions. Therefore, this work shows that the use of both polymeric membranes has shown high efficiency, and can be used in the purification of bromelain enzymes.
