Elevated Circulating Angiogenic Progenitors and White Blood Cells Are Associated with Hypoxia-Inducible Angiogenic Growth Factors in Children with Sickle Cell Disease
Solomon F. Ofori-Acquah,
Iris D. Buchanan,
Ifeyinwa Osunkwo,
Jerry Manlove-Simmons,
Feyisayo Lawal,
Alexander Quarshie,
Arshed A. Quyyumi,
Gary H. Gibbons,
Beatrice E. Gee
Affiliations
Solomon F. Ofori-Acquah
Department of Pediatrics, Division of Hematology/Oncology, Emory University School of Medicine, 2015 Uppergate Dr. NE, Atlanta, GA 30322, USA
Iris D. Buchanan
Department of Pediatrics, Morehouse School of Medicine, 720 Westview Drive, SW Atlanta, GA 30310-1495, USA
Ifeyinwa Osunkwo
Department of Pediatrics, Division of Hematology/Oncology, Emory University School of Medicine, 2015 Uppergate Dr. NE, Atlanta, GA 30322, USA
Jerry Manlove-Simmons
Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive, SW Atlanta, GA 30310-1495, USA
Feyisayo Lawal
Morehouse College, 830 Westview Dr SW, Atlanta, GA 30314, USA
Alexander Quarshie
Biostatistics Core, Morehouse School of Medicine, 720 Westview Drive, SW Atlanta, GA 30310-1495, USA
Arshed A. Quyyumi
Department of Medicine, Division of Cardiology, 1462 Clifton Road N.E. Suite 507, Atlanta, GA 30322, USA
Gary H. Gibbons
Cardiovascular Research Institute, Morehouse School of Medicine, 720 Westview Drive, SW Atlanta, GA 30310-1495, USA
Beatrice E. Gee
Department of Pediatrics, Morehouse School of Medicine, 720 Westview Drive, SW Atlanta, GA 30310-1495, USA
We studied the number and function of angiogenic progenitor cells and growth factors in children aged 5–18 years without acute illness, 43 with Hemoglobin SS and 68 with normal hemoglobin. Hemoglobin SS subjects had at least twice as many mononuclear cell colonies and more circulating progenitor cell than Control subjects. Plasma concentrations of erythropoietin, angiopoietin-2, and stromal-derived growth factor (SDF)-1α were significantly higher in children with Hemoglobin SS compared to Control subjects. In a multivariate analysis model, SDF-1α concentration was found to be associated with both CPC number and total white blood cell count in the Hemoglobin SS group, suggesting that SDF-1α produced by ischemic tissues plays a role in mobilizing these cells in children with Hemoglobin SS. Despite having a higher number of angiogenic progenitor cells, children with Hemoglobin SS had slower migration of cultured mononuclear cells.
