Use of Three Air Samplers for the Detection of PRRSV-1 under Experimental and Field Conditions
Heiko Stein,
Jochen Schulz,
Rebecca Morgenstern,
Thomas Voglmayr,
Georg Freymüller,
Leonie Sinn,
Till Rümenapf,
Isabel Hennig-Pauka,
Andrea Ladinig
Affiliations
Heiko Stein
Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, 1210 Vienna, Austria
Jochen Schulz
Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany
Rebecca Morgenstern
Boehringer Ingelheim RCV GmbH & Co KG, 1120 Vienna, Austria
Thomas Voglmayr
Traunkreis Vet Clinic, 4551 Ried im Traunkreis, Austria
Georg Freymüller
Traunkreis Vet Clinic, 4551 Ried im Traunkreis, Austria
Leonie Sinn
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria
Till Rümenapf
Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, 1210 Vienna, Austria
Isabel Hennig-Pauka
Field Station for Epidemiology, University of Veterinary Medicine Hannover, Foundation, 49456 Bakum, Germany
Andrea Ladinig
Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine Vienna, 1210 Vienna, Austria
Airborne transmission of porcine reproductive and respiratory syndrome virus (PRRSV) has been known for a long time. Most experiments were performed using PRRSV-2 strains and fairly little information is available on the airborne spread of PRRSV-1. The aim of this study was to assess three different air samplers for their ability to detect PRRSV-1 under experimental and field conditions. All three devices were able to detect PRRSV-1 by quantitative reverse trascription polymerase chain reaction (qRT-PCR) under experimental conditions. However, the detection of PRRSV-1 in a PRRSV-positive farm with active virus circulation was not successful.
