Rapid detection of iprodione in cucumber and apple using an immunochromatographic strip test

Abstract

In recent years, fungicides have been frequently detected in soil, water, and air because of their extensive use, with detrimental effects on the environment. Therefore, the screening of foods for iprodione (IPRO) has become mandatory. A rapid, specific and sensitive method based on an immunochromatographic strip test has been developed for the determination of IPRO residues in cucumber and apple. The antigen IPRO-ovalbumin and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the test and control lines, respectively. Under optimal conditions, the cut-off limits of the semi-quantitative strip test for IPRO was as low as 10 ng/mL in 0.01 M PBS pH 7.4, 20 ng/mL in cucumber juice, and 25 ng/mL in apple juice and the results were obtained within 5 min. Our data suggests that this immunochromatographic assay can be used for the sensitive, rapid, and specific on-site screening of IPRO in cucumber and apple.

Keywords

• fungicides
• immunochromatographic assay
• iprodione
• monoclonal antibody
• strip test