Effect of direct adrenaline infusion into isolated rat heart on the induction of ventricular tachyarrhythmias
Ahmet Davut Aksu,
Jana Hložková,
Gerardo Enrique Abarca Ríos,
Mohammed Naeem Malek,
Roman Panovský,
Grażyna Groszek,
Petr Mokrý,
Tomáš Kepák,
Peter Scheer,
Milan Sepši
Affiliations
Ahmet Davut Aksu
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic; International Clinical Research Centre, St. Anne's University Hospital, Brno, Czech Republic; Correspondence author.
Jana Hložková
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic; International Clinical Research Centre, St. Anne's University Hospital, Brno, Czech Republic
Gerardo Enrique Abarca Ríos
Department of Pharmacology, Pharmacy Faculty, University of Costa Rica, San Pedro, Costa Rica
Mohammed Naeem Malek
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic
Roman Panovský
Department of Internal Cardio-angiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Grażyna Groszek
Faculty of Chemistry, Rzeszow University of Technology, Rzeszow, Poland
Petr Mokrý
Department of Chemical Drugs, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic
Tomáš Kepák
Department of Paediatric Oncology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Peter Scheer
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Masaryk University, Brno, Czech Republic; International Clinical Research Centre, St. Anne's University Hospital, Brno, Czech Republic
Milan Sepši
Department of Internal Cardiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
• In this study, hearts from 72 male Wistar albino rats were divided into two main protocols: a 40 min ischemia group (protocol A, n = 53) and 10 min ischemia group (protocol B, n = 19). Protocol A subdivided into 2 groups as a control group (n = 10) and adrenaline group (n = 43). Protocol B is subdivided into 2 groups as control group (n = 10) and adrenaline group (n = 9). Both adrenaline groups received the same dose of adrenaline. • In protocol A, ventricular tachyarrhythmia (VTA) incidence was 0 % in controls but rose to 72 % in the adrenaline group (p < 0.01). Heart rates for the control and adrenaline groups in stabilization and reperfusion were 254±45 bpm and 247 ± 66 bpm, versus 277 ± 41 bpm and 651 ± 286 bpm, respectively. • In protocol B, VTA incidence reached 100 % in both groups during reperfusion, with heart rates of 393 ± 29 bpm and 892±227 bpm for controls and 350 ± 49 bpm and 949 ± 116 bpm for the adrenaline group. • These findings suggest that direct adrenaline administration into the heart in last 5 mins of the ischemic period and the 5 mins of in the reperfusion time increases the incidence of reperfusion-induced ventricular arrhythmias up to 72 % in protocol A. Protocol B hearts showed reperfusion-induced ventricular arrhythmias with 100 % incidence in both groups.
